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10 Best Enzyme Cleaners for Dog Urine in 2021 – Reviews & Top Picks

Simple Green

If you have a dog who has urinated on the floor or the side of your couch to mark their territory, you know that it can be difficult to remove the residual urine, as well as the odor. You may have cleaned the area, yet your dog continues to urinate in that particular spot.

Did you know that a dog’s nose has 300 million olfactory receptors compared to our measly 6 million? So, while we may think that the smell is gone, the dog knows it’s still there. That’s why it’s important to find an enzyme cleaner that will get rid of the odor once and for all.

This review list of the top 10 enzyme cleaners for dog urine will help reduce the frustration of deciding which cleaner to choose. Our buyer’s guide will give you tips and considerations to keep in mind when you buy a cleaner, so you can find the best one for your situation.

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A Quick Comparison of Our Favorites for 2021

Image Product Details
Best Overall
Simple Solution Enzymatic Cleaner Simple Solution Enzymatic Cleaner
  • Feature iconWorks on a variety of messes
  • Feature iconReduces occurrences (urinating)
  • Feature iconThree-in-one sprayer
  • Best Value
    Second place
    OUT! Advanced Stain & Odor Remover OUT! Advanced Stain & Odor Remover
  • Feature iconUse on many messes
  • Feature iconRemoves old stains
  • Feature iconPrevents further urinating
  • Premium Choice
    Third place
    SIMPLE GREEN Active Stain Enzyme Cleaner SIMPLE GREEN Active Stain Enzyme Cleaner
  • Feature iconNon-toxic
  • Feature iconRemoves a variety of stains
  • Feature iconGreat at eliminating odors
  • Rocco & Roxie Stain & Odor Eliminator Rocco & Roxie Stain & Odor Eliminator
  • Feature iconGreat for carpets
  • Feature icon100% guarantee
  • Feature iconWorks on a variety of surfaces
  • BUBBAS Commercial Enzyme Cleaner BUBBAS Commercial Enzyme Cleaner
  • Feature iconNatural dormant bacteria
  • Feature iconNon-chlorine formula
  • Feature iconGreat for carpets
  • The 10 Best Enzyme Cleaners for Dog Urine

    1. Simple Solution Enzymatic Cleaner — Best Overall

    Simple Solution 10137N-12PONL Enzymatic Cleaner

    Our top choice for an enzymatic cleaner is from Simple Solution because it provides great cleaning power for a variety of pet messes. It is an affordable option that works well on feces, urine, vomit, and drool, all of which are issues that you face when you have a dog. It is safe to use on any surface that can be cleaned with water.

    We like the three-in-one sprayer, as it allows you to choose the way to release the solution depending on the type of stain or odor to be removed. The foam spray is best for embedded stains since it will stay where you spray it, allowing it to break down the stain. The stream option is more accurate and will get into hard-to-reach areas while providing great odor control. Last is the mist, which is perfect for surface stains since it covers a large area in one shot.

    The Simple Solution is made up of pro-bacteria and enzymes that dissolve stains, neutralize odors, and help prevent repeated occurrences (such as urinating in one particular area). If used as directed, it is safe around pets and children. We found that it works best on newer stains, rather than old, set-in stains.

    • Works on a variety of messes
    • Reduces occurrences (urinating)
    • Three-in-one sprayer
    • Affordable
    • Safe around pets and children
    • Works best on new stains
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    2. OUT! Advanced Stain & Odor Remover — Best Value

    OUT 70674-4P Advanced Stain and Odor Remover

    This is the best enzyme cleaner for dog urine for the money since it has three times the concentration of pro-bacteria and enzymes and works well to remove pet stains and odor. This enzymatic cleaner is ideal for stains related to urine, vomit, feces, and blood and can be used on any water-safe surface.

    We like that you can use it on laundry as a pre-wash and that it can be added to your carpet-cleaning machine for extra cleaning power. It will also eliminate pheromones that are released when your dog urinates, to prevent further visitations to the same area.

    It is safe to use around children and pets when the directions are followed. It does a good job of releasing older stains, but the odor of the solution may be strong for some people. This did not make the number-one spot since it doesn’t remove pet odors as well as the Simple Solution.

    • Affordable
    • Use on many messes
    • Removes old stains
    • Prevents further urinating
    • Safe around children and pets
    • Strong odor
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    3. SIMPLE GREEN Active Stain Enzyme Cleaner — Premium Choice

    SIMPLE GREEN 2070700100002 Bio Dog Active Stain Enzyme Cleaner

    This is a non-toxic formula designed to eliminate tough stains and odors left by your dog. It will work on urine, feces, vomit, dirt, and blood. It also does well to get rid of scent markings so your dog won’t urinate in the same area again.

    Simple Green Enzyme Cleaner is made from natural enzymes and can be used on any porous surface, such as rugs, bedding, clothing, and carpets. It will also work on linoleum, tile, wood, laminates, and many other hard surfaces. We like that the formula penetrates deep into the fabric to release stains and doesn’t leave any residual odor or residue.

    This cleaner is made in the U.S.A., and the company backs its product with a 100% guarantee so you can return it if you are not satisfied. The Simple Green is pricier than the Simple Solution and the OUT!, which is why we rated it as our third choice on the reviews list.

    • Non-toxic
    • Removes a variety of stains
    • Great at eliminating odors
    • Can use on a variety of surfaces
    • 100% guarantee
    • Pricey
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    4. Rocco & Roxie fsec Stain & Odor Eliminator

    Rocco & Roxie

    This stain remover is considered professional-grade and works especially well on carpets. It is certified by the Carpet and Rug Institute to be gentle and safe for all colorfast carpets. The company is a small family-run business that stands by their product and offers an 100% guarantee. It can be used on a variety of other surfaces, such as hardwood, tile, and litter boxes.

    It is made from natural enzymatic bacteria that eliminate odors and stains. We like that it is chlorine-free and safe to use around pets and children. On the downside, it does take extra time to clean with this product. Once you spray it on the area to be treated (one with a deep odor problem), it is recommended that you leave it in place for at least one hour, and multiple treatments may be needed to effectively remove the stain.

    • Great for carpets
    • 100% guarantee
    • Works on a variety of surfaces
    • Safe around pets and children
    • Requires long treatment time
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    5. BUBBAS Commercial Enzyme Cleaner


    This product uses natural dormant bacteria that are activated by the ammonia in the urine, and they are more effective and last longer since they have been resting for so long. This cleaner can be used for other stains, such as wine, and as a pre-wash treatment on your clothes.

    It is certified by the Carpet & Rug Institute, and we found it works best at removing urine stains on the carpet. The instructions are detailed, but on the downside, there are many steps to clean an old stain. You have to apply water to the carpet to soak it to the padding, let it sit for five minutes, dry the excess water, soak the carpet with cleaner, and then cover with a towel and let it sit overnight.

    The price is somewhat affordable, but since you have to use a large amount to treat a stain, you will go through high amounts of the product quickly if you have many areas to treat. On the upside, it does come with a 100% guarantee.

    • Natural dormant bacteria
    • Non-chlorine formula
    • Great for carpets
    • 100% guarantee
    • Affordable
    • Lengthy cleaning process
    • Expensive if many areas to clean
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    6. Nature’s Miracle Stain & Odor Remover

    Nature’s Miracle P-96963 Stain and Odor Remover

    Nature’s Miracle is an affordable enzymatic formula that is formulated to work fast at removing dirt and debris. It does well at breaking up new stains and removing them immediately on contact. You can use this for urine, feces, drool, and vomit. It is safe to use on carpets, hard floors, and fabrics.

    We like the light citrus scent that isn’t overpowering, and it does well at eliminating the odor from dog urine. But on the downside, it doesn’t get rid of the actual stain. Unfortunately, the design of the spray nozzle on this product is also poor, and it is difficult to remain accurate since it has a wide spray area.

    It is safe to use around pets and children, and the company offers a money-back guarantee if you are not satisfied with the product.

    • Works fast
    • Best for new stains
    • Safe for a variety of surfaces
    • Safe around pets and children
    • Money-back guarantee
    • Poor nozzle design
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    7. ANGRY ORANGE Enzyme Stain Cleaner

    ANGRY ORANGE Enzyme Stain Cleaner

    A unique feature with this enzymatic cleaner is that it will work for up to 80 hours after applying — the enzymes are activated on contact and begin working at killing odor and fighting the stain. It is safe to use around pets and children and can be applied to carpets, litter boxes, furniture, and kennels.

    It offers a pleasant orange smell that is not overpowering, but it leaves an oily residue behind and the smell dissipates rapidly. Soap and water will remove the residue, though some may not want to take this extra step.

    It is pricier than others on our list, but it does well at removing old stains and odors within the carpet. Angry Orange offers a 100% satisfaction guarantee, and the company will refund your money in a timely manner.

    • Long-acting formula
    • Safe around pets and children
    • Can apply to a variety of surfaces
    • 100% satisfaction guarantee
    • Pleasant scent
    • Scent dissipates rapidly
    • Oily residue
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    8. Emmy’s Best Powerful Pet Odor Remover

    Emmy's Best Powerful Pet Odor Remover

    This triple-action formula is made to get rid of stains, neutralize odors, and remove pheromones to prevent repeat soiling. We like that this cleaner removes the odor instead of masking it with its lavender scent. It will work on a variety of surfaces, from carpets to concrete floors, to remove urine, feces, and other pet messes.

    The 32-ounce spray bottle is pricier than some, and we found it does not completely remove a stain nor deter your pet from urinating in the same area as effectively as other products on our list.

    It does work fast to neutralize the odor, and the smell is pleasant, calming, and not overpowering. Emmy’s offers a satisfaction guarantee on this product, so if you aren’t satisfied, you can contact them for a refund.

    • Triple-action formula
    • Removes odors effectively
    • Pleasant scent
    • Use on a variety of surfaces
    • Doesn’t fully remove stains
    • Not effective at deterring
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    9. Amaziing Solutions Pet Odor & Stain Remover

    Amaziing Solutions FBA_15806 Pet Odor and Stain Remover

    This cleaner is specifically designed to use on carpets and upholstery and is safe around children and pets. It is made in the U.S.A. and contains natural enzymes to neutralize odors and dissolve stains. It is certified by the Carpet and Rug Institute and won’t discolor your carpets.

    The instructions for Amaziing Solutions are detailed and require many steps to remove an old stain. We also found that repeated applications are necessary and that you will have to go through a high amount of this cleanser to see results. It doesn’t do well to prevent your dog from urinating in the same area, but it does neutralize odors from a human standpoint.

    • Certified
    • Safe around pets and children
    • Specific for carpets and fabric
    • Many steps to clean stains
    • Repeated applications required
    • Doesn’t prevent repeat soiling
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    10. Smiling Paws Pets Urine Enzyme Cleaner

    Smiling Paws Pets Pet Urine Enzyme Cleaner

    The Smiling Paws cleaner is made from an advanced bio-enzyme blend that can be used on a variety of surfaces, such as carpet, rugs, and hardwood floors. It is ideal for removing pet stains and odors due to urine, feces, and vomit.

    This cleaner has a non-toxic formula that is safe to use around pets, and the family-run company offers a satisfaction guarantee — if you don’t like the product, they will refund your money. On the downside, the spray nozzle is ineffective and cannot be adjusted to make the stream more accurate.

    The smell of this product is an overpowering floral scent and does not dissipate very fast, so it masks the smell but doesn’t eliminate the odor, nor does it prevent repeat soiling.

    • Bio-enzyme blend
    • Use on a variety of surfaces
    • Non-toxic
    • Ineffective spray nozzle
    • Overpowering scent
    • Doesn’t eliminate urine odor
    • Doesn’t prevent repeat soiling
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    Buyer’s Guide

    As you search for an enzyme cleaner for dog urine, there are a few considerations to keep in mind to help you pick the ideal one for your situation. Remember that dogs have a stronger sense of smell than humans, and if any scent/pheromones remain, they will still be drawn to that area. So, finding the best enzyme cleaner will guarantee that you won’t have problems in the future.



    You should know what the cleaner is made from — if you will be using it around pets and children, it should be non-toxic and shouldn’t contain ammonia and/or bleach. In fact, solutions that contain those two ingredients can actually worsen the problem of your dog urinating in a particular area.

    The major ingredient should be the enzymes that break down the protein present in the stain. Most are eco-friendly and have a neutral pH level, which helps fight odors. Certain enzymes continue to work when allowed to stay on the surface for a long period of time.

    Many have fragrances to provide a fresh smell; your personal preference will determine the type of fragrance you choose.


    This includes how many surfaces you can use it on, as well as what types of messes it will clean up. If you have a dog who urinates on the carpet, find a cleaner formulated for carpets. If the couch is the go-to-spot, then a cleaner formulated for upholstery would be ideal.

    Ease of Use

    This is important, especially if you don’t have a great deal of time on your hands. Some formulas are made to work quickly and are best for newer stains and messes, while other solutions that have to sit for long periods of time will work better at dissolving old stains.

    Smiling Paws


    Price may or may not be an issue, but keep in mind how often you’ll have to use the cleaner and how much is used during one cleaning session. If you have to apply it multiple times to remove one stain, it can get pricey fast, since you have to buy larger quantities. Conversely, if it is a great cleanser and does the job the first time around, you can splurge on a pricier version.

    Tips for using enzyme cleaners:

    • It’s best to use them as soon as the mess happens.
    • Read and follow the directions for the best results.
    • Test the cleaner on a small area first to make sure it doesn’t damage the surface.
    • You can use a UV light to see where the urine is located and to know if any remains after cleaning.

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    Final Verdict

    You love your dog, but when messes happen, it can be difficult to remain positive when your house smells like urine. Using an enzyme cleaner for urine removal can help save your home and your sanity, so you can get rid of the odor and stains.

    The best overall cleaner to remove dog urine is from Simple Solution, which offers a three-in-one sprayer that can be customized to the type of mess you are cleaning. The OUT! cleaner is the best value since it is affordable while still able to clean tough stains and prevent your dog from urinating in the same area. If the price isn’t a concern, then Simple Green has a great option for removing strong odors and set-in stains while providing a non-toxic formula.

    We hope our reviews list and buyer’s guide for best enzyme dog urine removers helps you find the best enzyme cleaner that is easy to use, is within your budget and gets the job done, so you can enjoy the benefits of owning a dog and not have to worry about unsightly stains and awful odors.